Journal: PLoS ONE

Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

doi: 10.1371/journal.pone.0271746

Figure Lengend Snippet: Immunization plan in the experimental ducks.

Article Snippet: MoDCs were then stained with Rabbit FITC-H9N2 HA protein monoclonal antibody (Sino Biological Inc. Beijing, China) at 4°C for 1 h. Then washed twice with PBS, and re-suspended in 4% paraformaldehyde for flow cytometry (FACSAria III, BD Biosciences).

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck

doi: 10.1371/journal.pone.0271746

Figure Lengend Snippet: (A) Representative flow cytometry profile of the positive rates of MoDCs catching H9N2 IAIV antigen. From group a to group h are RPMI-1640 mock control group, H9N2 IAIV group, pam2CSK4-H9N2 IAIV group, poly (I:C)-H9N2 IAIV group, imiquimod-H9N2 IAIV group, pam2CSK4-imiquimod-H9N2 IAIV group, poly (I:C)-imiquimod-H9N2 IAIV group and pam2CSK4-poly (I:C)-imiquimod-H9N2 IAIV group, respectively. The data presented here are results from one experiment of three flow cytometry experiments. (B) The statistical graph of the positive rates of MoDCs catching H9N2 IAIV antigen. The data were analyzed using Flowjo7.6 software. Data represent the mean ±SEM of three independent experiments. Different letters (a~c) mean significant difference ( P <0.05); ab means has no significant difference between adjacent a or b ( P >0.05).

Article Snippet: MoDCs were then stained with Rabbit FITC-H9N2 HA protein monoclonal antibody (Sino Biological Inc. Beijing, China) at 4°C for 1 h. Then washed twice with PBS, and re-suspended in 4% paraformaldehyde for flow cytometry (FACSAria III, BD Biosciences).

Techniques: Flow Cytometry, Software

Journal: Molecular Medicine Reports

Article Title: KCTD1 mutants in scalp-ear-nipple syndrome and AP-2α P59A in Char syndrome reciprocally abrogate their interactions, but can regulate Wnt/β-catenin signaling

doi: 10.3892/mmr.2020.11457

Figure Lengend Snippet: Interaction between KCTD1 and AP-2α mutants in vivo . 293 cells were transiently transfected with expression vectors Myc-AP-2α and Myc-KCTD1 or their mutants for 30 h. (A) Co-IP of wild-type AP-2α and wild-type KCTD1 or KCTD1 mutants including A30E and P20S. (B) Co-IP of wild-type AP-2α and KCTD1 mutants including G62D, D69E and H74P. (C) Co-IP of wild-type AP-2α and KCTD1 mutants including P31H, P31R and P31L. (D) Co-IP of wild-type KCTD1 and AP-2α mutants including P59A, P60A, P61R and 4A. Whole-cell extracts were analyzed using mouse monoclonal anti-Myc antibody to confirm protein overexpression and were immunoprecipitated with rabbit polyclonal antibody against KCTD1. Immunoprecipitates were detected by western blotting using mouse monoclonal anti-Myc tag antibody. Pre-immune rabbit IgG served as the negative control. Quantification of bands was analyzed using ImageJ software. Ratio of AP-2α/KCTD1 indicates the amount of immunoprecipitated AP-2α proteins relative to the amount of immunoprecipitated KCTD1 proteins. IP, immunoprecipitation; KCTD1, potassium-channel tetramerization-domain-containing 1.

Article Snippet: Primary antibodies included Anti-Myc mouse monoclonal antibody (clone. no. 9E10; Santa Cruz Biotechnology, Inc.) and anti-HA rabbit polyclonal antibody (clone no. Y-11) (Santa Cruz Biotechnology, Inc.) at 1:200 dilution for 1 h at 4°C.

Techniques: In Vivo, Transfection, Expressing, Co-Immunoprecipitation Assay, Over Expression, Immunoprecipitation, Western Blot, Negative Control, Software